Authors
Qian Cui, Jiaxin Zheng, Yuanqing Zhang, Xiaowei Wu, Ting Bao, Wei Wen, Xiuhua Zhang, Shengfu Wang
Published in
Analytica chimica acta. Volume 1423. Pages 346144. Nov 15, 2026. Epub Aug 18, 2026.
Abstract
Thermal transformation of metal organic frameworks (MOFs) yields carbon-based catalysts with high activity and robust stability, providing an appealing strategy to enhance the performance of electrochemical sensing platform. Herein, by one-step pyrolysis of converting Fe-doped zeolitic imidazolate framework-8 into nitrogen-doped carbon-supported monodisperse Fe (Fe-N-C) catalysts, a dual-readout electrochemical biosensor was constructed for sensitive detection of vascular endothelial growth factor (VEGF). The stable FeNX coordination structure endowed the Fe-N-C catalysts with peroxidase-like activity via Fe(II) to Fe(III) conversion. Using Fe-N-C catalysts as amplified signal labels, the chronoamperometric curve and impedance signal of the proposed biosensor could be flexibly readout by adjusting the catalytic substrate of tetramethylbenzidine-hydrogen peroxide (TMB-H2O2) and 3,3'-diaminobenzidine-H2O2 (DAB-H2O2). Based on the biotin-streptavidin conjugation and aptamer-target recognition, the presence of target VEGF165 induced the released of Fe-N-C catalysts and the decrease of signal readout, achieving sensitive detection of VEGF165 with the detection limits of 22.9 pg mL-1 (current readout) and 20.5 pg mL-1 (impedance readout), respectively, holding promising potential for disease diagnosis and therapeutic monitoring.
PMID:
42838657
Bibliographic data and abstract were imported from PubMed on 07 Oct 2026.
Read full publication at:
Please sign in
to see all details.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 13
- Comments 0