Aptamer/magnetic-MIP dual-recognition sandwich biosensor for rapid and recyclable determination of kanamycin in food.

Authors

Tingting Wang, Fengjuan Zhao, Zhiguang Suo, Yong Liu, Yawei Huang, Min Wei

Published in

Analytica chimica acta. Volume 1423. Pages 346165. Nov 15, 2026. Epub Aug 27, 2026.

Abstract

The rapid and precise monitoring of antibiotic residues is a cornerstone of food safety and diagnostic science. Herein, we report a high-performance dual-recognition sandwich-type fluorescent biosensor engineered by integrating Fe₃O₄@UiO-66-based molecularly imprinted polymers (FUMIP) with a fluorescein-labeled aptamer probe (Apt-FAM) for the ultrasensitive detection of kanamycin (KANA).
Central to this design is the confined microenvironment provided by the FUMIP framework, which facilitates the selective pre-concentration of KANA through coordinated boronate affinity and hydrogen bonding. This interfacial enrichment significantly facilitates subsequent recognition by the Apt-FAM probe, forming a stable sandwich architecture that achieves a 156-fold sensitivity enhancement compared with the single-recognition control system constructed in this work. The FUMIP component exhibits a superior imprinting factor of 5.19 and an adsorption capacity of 45.11 mg/g, ensuring high-fidelity target capture even in complex matrices. The optimized biosensor enables rapid quantification within 30 min, delivering an exceptional detection limit of 0.0032 ng/mL across a broad linear range (0.01-1000 ng/mL). In comparison, the single-recognition sensor exhibits a linear range of 1-500 ng/mL with a detection limit of 0.5010 ng/mL. Furthermore, the sensor demonstrates robust reusability (70% signal retention after 20 cycles) and high practical reliability, with recoveries of 98.0%-106.2% in real food samples.
This work not only provides an advanced platform for KANA monitoring but also offers a universal strategy for the ultrasensitive detection of small-molecule contaminants by exploiting the synergy between synthetic and biological receptors.

PMID:
42838675
Bibliographic data and abstract were imported from PubMed on 07 Oct 2026.

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