Simultaneous Infection With Genetically Distinct Trypanosoma cruzi Isolates Is Associated With Distinct Myocardial Innate Immune Transcriptional Profiles During Acute Infection in Mice.

Authors

George Harisson Felinto Sampaio, Andressa Noronha Barbosa-Silva, Carlos Ramon do Nascimento Brito, Lucas Abrantes Batista, Christiane Carlos Araújo de Negreiros, Ramayana Morais de Medeiros Brito, Rand Randall Martins, Manuela Sales Lima Nascimento, Antonia Claudia Jácome da Câmara, Paulo Marcos da Matta Guedes, Lúcia Maria da Cunha Galvão

Published in

Parasite immunology. Volume 48. Issue 10. Pages e70103.

Abstract

Innate immune receptors are central to host resistance against Trypanosoma cruzi infection, yet how parasite genetic diversity is associated with these responses remains poorly understood. Here, we characterized acute infection outcomes and myocardial innate immune transcriptional profiles in mice subjected to single and mixed infections with genetically distinct T. cruzi parasites (DTUs TcII, TcIII, and TcVI). Swiss mice were infected with RN2, RN23, Y, or CL-Brener individually or in combination, and parasitemia, survival, and cardiac gene expression were assessed. The Y strain, CL-Brener clone, and RN2 + RN23 + CL-Brener condition exhibited a high-severity profile, with marked parasitemia (8000-28,000 trypomastigotes/5 μL), mortality of 74%-100%, reduced myocardial transcription of Tlr2, Tlr4, Tlr9, Trif, and Myd88, and increased transcription of Nlrp3, Casp1, Il1β, and Tnfα. In contrast, RN2 and RN23 single infections exhibited low-severity profiles and higher transcription of innate immune mediators associated with parasite control. Mixed-infection groups displayed transcriptional and infection profiles differing from the corresponding single-infection groups under a standardized total inoculum. Together, these findings identify distinct myocardial innate immune transcriptional profiles across single and mixed T. cruzi infection conditions and support further investigation of the contributions of parasite population composition and dose to acute infection outcomes.

PMID:
42839890
Bibliographic data and abstract were imported from PubMed on 07 Oct 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.