Authors
Carolin Gierse, Ingrid R Vetter, Donna Schweizer, Leif Dehmelt
Published in
Life science alliance. Volume 9. Issue 12. Epub Oct 07, 2026.
Abstract
The spatiotemporal regulation of cell contraction is crucial for numerous biological processes. In particular, local, subcellular contraction pulses in the cell cortex contribute to mechanotransduction and tissue rearrangements during embryonic development. We previously identified a signaling network that generates mechanosensitive contraction pulses in adherent mammalian cells through positive and negative feedback regulation of the small GTPase RhoA. Here, we show that ROCK1 is rapidly recruited to cortical pulsatile Rho activity zones and that efficient recruitment is synergistically mediated by the canonical Rho-binding domain and the C-terminal PHC1 domain. Surprisingly, the isolated ROCK1 PHC1 domain by itself is already sufficient for dynamic recruitment to active Rho signaling zones in cells, and AlphaFold-guided mutagenesis identifies conserved PHC1 residues that are required for this recruitment. Functional investigations show that the PHC1 domain plays an important role in transduction of Rho activity to myosin activation. Together, our data revise models of ROCK1 regulation by identifying a C-terminal PHC1 domain-dependent mechanism that contributes to ROCK1 recruitment and signal transduction during cell contraction pulses.
PMID:
42843975
Bibliographic data and abstract were imported from PubMed on 08 Oct 2026.
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