Authors
Parvin Mohammadi, Mina Habibizadeh, Zhila Izadi, Kamran Mansouri, Mahmoudreza Moradi
Published in
Journal of biomedical materials research. Part A. Volume 114. Issue 10. Pages e70166.
Abstract
Urethral stricture remains a challenging clinical condition, particularly in patients with long or complex defects where suitable donor tissue is limited. Tissue engineering offers a promising alternative through the development of biomimetic scaffolds capable of restoring urethral structure and function. In this study, we fabricated a sandwich nanofibrous scaffold based on polyglycolic acid (PGA) and decellularized urethral extracellular matrix (uECM) using a sandwich design (uECM/PGA/uECM) and compared it with PGA and mix (PGA-uECM) scaffolds. Successful decellularization was confirmed by histological and molecular analyses, demonstrating effective removal of cellular components while preserving ECM architecture. Structural characterization revealed high porosity, enhanced hydrophilicity, favorable swelling behavior, and controlled degradation in ECM-containing scaffolds. The uECM/PGA/uECM construct exhibited superior mechanical strength while maintaining suitable elasticity for urethral applications. Biological evaluation showed significantly improved endothelial cell adhesion, cell viability, and migration in ECM-integrated scaffolds compared with PGA alone. In vivo implantation demonstrated excellent biocompatibility, absence of adverse inflammatory responses, and evident neovascularization. Overall, the sandwich uECM/PGA/uECM scaffold combines mechanical stability with bioactive functionality, highlighting its potential as a promising candidate for urethral tissue regeneration.
PMID:
42844943
Bibliographic data and abstract were imported from PubMed on 08 Oct 2026.
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