Authors
Chamaiporn Supachettapun, Titilope John Jayeoye, Preeyawan Wongsam, Maiko K Okajima, Tatsuo Kaneko, Paisan Khanchaitit, Kanokwan Sansanaphongpricha, Kazuaki Matsumura, Nongnuj Muangsin
Published in
Journal of materials chemistry. B. Oct 09, 2026. Epub Oct 09, 2026.
Abstract
Liposomes (LPs) are widely used in biomedical applications but suffer from poor physiological stability, dose-dependent cytotoxicity, and rapid clearance. To overcome these challenges, we developed quaternized hyaluronate-coated cationic liposomes (QHA-LPs) for enhanced stability and cell interactions. QHA-LPs are polyampholytic, carrying both positive charges introduced through quaternization and the native negative charges retained from the HA backbone. This quaternization strategy produced QHA-LPs (∼140 nm) with significantly improved colloidal stability compared to native HA-LPs. Consequently, the QHA-LPs maintained colloidal stability, retained >90% CRL2522 fibroblast viability at concentrations up to 200 µg mL-1, achieved 92.3 ± 7.5% DOX encapsulation efficiency, and showed more than two-fold higher MCF-7 uptake compared to uncoated LPs and HA-LPs. In MCF-7 cells, the DOX-loaded QHA-LPs showed the lowest IC50 (0.17 µM) among all formulations tested, approximately three-fold lower than that of free DOX (0.50 µM). In A549 cells, all liposomal formulations showed lower IC50 values than free DOX, with NaHA-LPs/DOX showing the lowest IC50 (0.12 ± 0.06 µM), followed by QHA-LPs/DOX (0.16 ± 0.05 µM), consistent with CD44-mediated and charge-mediated uptake in a cell-type-dependent manner. These in vitro observations demonstrated the stability of the QHA-LPs under the tested pH conditions for carrier DOX delivery, though this remains to be evaluated in vivo.
PMID:
42853083
Bibliographic data and abstract were imported from PubMed on 09 Oct 2026.
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