Dual Host-Pathogen Screening of the Lung During Severe Influenza Using Spatial Transcriptomics.

Authors

Marcus Z W Tong, Oliver P Skinner, Chen Zhu, Brooke J Wanrooy, Ashlyn S Kerr, Zheng Ruan, Cameron G Williams, Heran Zhang, Linda M Wakim, Ashraful Haque

Published in

Immunology and cell biology. Oct 11, 2026. Epub Oct 11, 2026.

Abstract

Here, we assessed whether spatially resolved capture of polyadenylated mRNA permitted simultaneous screening of host and viral responses in situ. Fresh-frozen lung samples from mice infected with H1N1 influenza A strain, PR8, were processed via genome-wide, spatial transcriptomics (ST) at 10-μm resolution. To determine if specific cell-types were detectable within the ST data, we first performed cellular deconvolution using publicly available, single-cell RNA-seq data of immune and non-immune cells from PR8-infected lungs. This revealed expected structures such as rings of epithelial cells lining airways, and at Day 10 post-infection (p.i.) unexpected structures dominated by B cells. We next screened ST data alone for groups of genes displaying similar spatial expression patterns. This again identified at Day 10 p.i. cellular aggregates enriched for genes associated with B cell biology. Mapping these gene signatures to a B cell transcriptomic atlas suggested plasmablasts had emerged early in the lung, which was confirmed by flow cytometry. Finally, by mapping ST data to the influenza A genome, we observed widespread expression of all 10 viral transcripts across samples at Day 4 but not Day 10 p.i., consistent with viral clearance. Cellular deconvolution analysis at Day 4 p.i. revealed proportions of every detected cell-type harbored viral transcripts, suggesting indiscriminate infection across the specific area of tissue examined. Subsequent whole-section immunohistochemical assessment further supported the phenomenon of localized indiscriminate infection, with these areas found adjacent to minimally infected zones. Thus, spatial screening of mRNA in tissues can simultaneously inform upon viral pathogenesis and host responses to infection.

PMID:
42860245
Bibliographic data and abstract were imported from PubMed on 12 Oct 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.