Effect of Dermal Fibroblast-Conditioned Media on Human Dermal Fibroblasts Treated With Electromagnetic Transduction Therapy.

Authors

Jeongah Ko, Sang Bum Suh, Yeong Ju Oh, Ji Youn Suh, Sung Bin Cho

Published in

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI). Volume 32. Issue 10. Pages e70394.

Abstract

Electromagnetic transduction therapy (EMTT) is a high-intensity pulsed electromagnetic field therapy used in musculoskeletal medicine. However, its therapeutic effects on human dermal fibroblasts (HDFs) remain unclear. Secretomes from human dermal fibroblast-conditioned medium (HDFCM) can enhance wound repair and tissue regeneration.
Young and aged HDFs were treated with EMTT (40 or 80 mT) following HDFCM pretreatment (0, 30, or 60%). Proliferation and migration were assessed using cell proliferation and wound healing assays. Senescence-associated β-galactosidase (SA-β-Gal) staining and enzyme-linked immunosorbent assay (ELISA) were performed to evaluate the effects of EMTT and HDFCM on senescence and pro- and anti-inflammatory cytokine secretion.
HDFCM significantly enhanced the proliferation of young and aged HDFs, particularly aged cells, in a dose-dependent manner. EMTT at 40 mT further enhanced these effects, with 40 mT EMTT plus 30% HDFCM yielding the greatest increase in the number of aged HDF. SA-β-Gal activity, minimal in young cells, was elevated in aged HDFs, but was reduced by 40 mT EMTT with or without HDFCM. Contrastingly, 80 mT EMTT increased SA-β-Gal activity unless counteracted by 60% HDFCM. Moreover, interleukin-6 was upregulated and transforming growth factor-β was downregulated by HDFCM and EMTT treatments.
EMTT at low magnetic field strength, particularly if combined with HDFCM, promotes proliferation and migration while reducing senescence in aged HDFs. This approach may offer a promising therapeutic strategy for skin regeneration and antiaging applications, especially for aging or damaged dermal tissues.

PMID:
42861896
Bibliographic data and abstract were imported from PubMed on 12 Oct 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.