Condensation of MATCAP promotes CENP-E-dependent chromosome congression in mitosis.

Authors

Tongtong Yang, Wenping Hu, Panpan Xu, Huanyu Li, Yaqian Zhang, Fangyuan Xiong, Yunze Li, Chao Xu, Ke Ruan, Zhonghuai Hou, Zhikai Wang, Liangyu Zhang, Xuebiao Yao, Shengqi Xiang, Kai Jiang, Xing Liu

Published in

Nature communications. Volume 17. Issue 1. Jul 30, 2026. Epub Jul 30, 2026.

Abstract

Accurate segregation of mitotic chromosomes requires pre-anaphase alignment driven by microtubule-based motor proteins. Tubulin detyrosination is essential to guide CENP-E-driven chromosome congression in mitosis. However, the mechanisms of action and physiochemical properties of the detyrosinases for decoding CENP-E motility remain elusive. Here we show that microtubule-associated tyrosine carboxypeptidase (MATCAP) undergoes intrinsically disordered region (IDR)-dependent liquid-liquid phase separation (LLPS) on microtubules to constitute the tubulin detyrosination machinery. These biomolecular condensates selectively enrich tubulin and CENP-E, thereby stabilizing kinetochore-microtubule attachments. Real-time imaging of cells expressing LLPS-deficient MATCAP mutants reveals the importance of MATCAP LLPS dynamics in mitotic chromosome alignment. Mechanistically, phase separation of MATCAP spatiotemporally couples tubulin detyrosination with CENP-E motility to ensure a robust chromosome alignment during mitosis. These findings delineate a signaling cascade that integrates phase separation and tubulin detyrosination with mitotic progression for the maintenance of genomic stability.

PMID:
42660891
Bibliographic data and abstract were imported from PubMed on 13 Oct 2026.

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