Authors
Eri Kawashita, Kaneyasu Nishimura, Keiichi Ishihara, Eriko Sano, Suzuka Noguchi, Yuna Misaki, Kazuyuki Takata, Osamu Matsuo, Satoshi Akiba
Published in
Biological & pharmaceutical bulletin. Volume 49. Issue 10. Pages 1650-1658.
Abstract
Neural precursor cell (NPC) transplantation is a promising therapeutic strategy for reconstructing damaged neuronal circuits and achieving functional recovery in neurological diseases. However, the impact of the host microenvironment on the survival and maturation of transplanted cells is not fully understood. The fibrinolytic system regulates clot lysis and extracellular matrix remodeling through proteolytic activity, but its role in neuronal maturation following NPC transplantation remains to be elucidated. This study investigated whether host deficiency of α2-antiplasmin (α2AP), the primary inhibitor of plasmin, affects the engraftment and maturation of transplanted NPCs in the mouse brain. Green fluorescent protein-expressing mouse embryonic stem cell-derived NPCs were transplanted into the brains of wild-type (WT) and α2AP-knockout (KO) mice. Graft size and cell survival were comparable between WT and α2AP-KO mice, suggesting that host α2AP deficiency does not affect engraftment efficiency. In contrast, the number of neurites in transplanted neurons tended to be lower in α2AP-KO mice, particularly in DCX-positive immature neurons. These findings suggest that α2AP in the host environment may contribute to the morphological maturation of transplanted neurons rather than to their survival and differentiation.
PMID:
42863997
Bibliographic data and abstract were imported from PubMed on 13 Oct 2026.
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