Authors
Keita Yamamoto, Shiori Shikata, Yangying Hao, Takuya Tomita, Rina Kanai, Els Verhoeyen, Yasushi Saeki, Susumu Goyama
Published in
Nucleic acids research. Volume 54. Issue 18. Sep 22, 2026.
Abstract
RNA-protein interactions (RPIs) are as important as protein-protein interactions (PPIs) for the formation of membraneless organelles (MLOs) and play vital roles in diverse biological processes. Despite remarkable advances in PPI analysis technologies, the development of tools for RPI analysis has lagged behind. To address this challenge, we integrated three established PPI technologies-bimolecular fluorescence complementation (BiFC), NanoBiT, and split-TurboID-with RNA-targeting CRISPR/dCas13. We applied these platforms to paraspeckles, a representative MLO formed through interactions between the long non-coding RNA NEAT1 and the RNA-binding protein NONO. BiFC-dCas13 functions as a fluorescence-based recorder that enables quantitative detection of NEAT1-NONO interaction events. NanoBiT-dCas13 precisely monitors dynamic changes in the NEAT1-NONO interaction through a reversible luminescence readout. Split-TurboID-dCas13 induces proximity-dependent biotinylation of proteins surrounding paraspeckles, identifying the N6-methyladenosine reader YTHDC1 as a paraspeckle-associated protein. Furthermore, BiFC-dCas13 was readily reconfigured to detect additional RPI pairs, demonstrating the modularity of the platform. Together, these complementary systems provide versatile approaches for recording, monitoring, and discovering RPI-associated biology and molecular environments.
PMID:
42863841
Bibliographic data and abstract were imported from PubMed on 13 Oct 2026.
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