Monoallelic POLR3A Variants Cause Early-Onset Peripheral Neuropathy.

Authors

Luiza L P Ramos, Jevin M Parmar, Robin Wijngaard, Bianca R Grosz, Tamas Lazar, Ligia Mateiu, Steve Vucic, Kishore R Kumar, Dennis Yeow, Laura I Rudaks, Lonneke de Boer, Annemarie de Vreugd, David A Koolen, Thatjana Gardeitchik, Anita Cairns, Krishnan Iyengar, Fernando Kok, Fernanda Barbosa Figueiredo, Alzira Alves de Siqueira Carvalho, Luiz S Mageste Barbosa, Rodrigo Rezende Arantes, Tyler Rehbein, Jordan E Bontrager, Elizabeth P Wood, Janet E Sowden, Gavin Monahan, Meutia Kumaheri, Ivy Cuijt, Melina Ellis, Gonzalo Perez-Siles, Elyshia McNamara, Ronald van Beek, Celine B Meijers, Ivaylo Tournev, Stephan Zuchner, Shoshana J Wodak, Clara D M van Karnebeek, Nigel Laing, Liana N Semcesen, David A Stroud, David N Herrmann, Velina Guergueltcheva, Marina L Kennerson, Machteld M Oud, Gianina Ravenscroft, Ayse Candayan, Albena Jordanova

Published in

Annals of neurology. Volume 100. Issue 3. Pages 655-671. Epub Jun 08, 2026.

Abstract

Biallelic variants in genes encoding the RNA polymerase III complex (Pol III) cause a spectrum of neurological disorders primarily affecting the central nervous system. Monoallelic variants have been reported in the POLR3B subunit only, associated with neurodevelopmental disorder, epilepsy, and peripheral neuropathy. We describe a novel Pol III-related disorder caused by monoallelic variants in POLR3A and presenting primarily with peripheral neuropathy.
We performed clinical and genetic investigation of the affected families. Biophysical characterization of mutant proteins was performed in silico. Immunoblotting, interactomics, and transcriptomics characterization were done in cellular models.
We identified 11 patients across 8 unrelated families harboring heterozygous missense variants in POLR3A, occurring de novo or segregating in an autosomal-dominant fashion. The patients presented with an early-onset, progressive sensorimotor peripheral polyneuropathy with intermediate to demyelinating ranges of nerve conduction slowing, occasionally accompanied with neurological or non-neurological features. White matter abnormalities, characteristic for the biallelic Pol III-related disorders, where not observed in the brain magnetic resonance imaging (MRI) evaluations. The neuropathy-associated variants cluster in regions critical for Pol III activity. We observed mis-regulation of individual Pol III targets and global downregulation of tRNA pools in patient-derived cells. This Pol III dysfunction was not due to impaired POLR3A expression, subcellular localization, or subunit interactions.
Our findings expand the Pol III-related disease spectrum beyond the known biallelic phenotypes and establish POLR3A as a novel peripheral neuropathy-associated gene. This work highlights a central role of Pol III dysfunction in disease and reinforces the link between tRNA metabolism and peripheral neurodegeneration. ANN NEUROL 2026;100:655-671.

PMID:
42260910
Bibliographic data and abstract were imported from PubMed on 14 Oct 2026.

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