Dietary Pichia Guilliermondii Improves Intestinal Digestive Capacity and Barrier Integrity in Weaned Piglets Associated with Gut Microbiota-Related Glutathione Metabolism.

Authors

Honglin Yan, Lunjie Feng, Junhao Xiang, Hongyu Yi, Zhaoxi Xie, Areeba Amir, Yong Zhang, Pan Zhou, Jinbiao Zhao, Tiande Zou, Hui Diao

Published in

Probiotics and antimicrobial proteins. Oct 14, 2026. Epub Oct 14, 2026.

Abstract

Yeast-derived feed additives are increasingly used to support intestinal health after weaning, but dose-response evidence and mechanistic information for inactivated Pichia guilliermondii product (PG) in pigs remain limited. A total of 360 piglets (initial body weight, 6.30 ± 0.06 kg) received diets containing 0, 800, 1600, 2400, or 3200 mg/kg inactivated PG product for 28 days. Under the present experimental conditions, the 800 mg/kg PG group showed the greatest weight gain, final body weight, and nutrient digestibility of dry matter and crude protein among the tested dietary levels (P < 0.05). Accordingly, the control and 800 mg/kg PG (PGO) groups were selected for downstream phenotyping. Compared with controls, PGO group had increased intestinal villus height, digestive enzyme activities, nutrient transporter expression, goblet cell numbers, and tight-junction protein abundance, and reduced serum diamine oxidase activity (P < 0.05). The PGO group also showed higher systemic and intestinal antioxidant-related indices, accompanied by increased expression of SOD1, GPX4, and NRF2 (P < 0.05). Microbiota analysis showed enrichment of potentially beneficial taxa, including Blautia, Prevotellaceae_NK3B31_group, and Prevotella_9. Colonic metabolomics further revealed higher levels of glycine, serine, and L-cysteine, together with increased intestinal activities and gene/protein expression of glutathione-related enzymes/factors, including GCLM, GCLC, and GSS (P < 0.05). These findings indicate that, under the present experimental conditions, 800 mg/kg inactivated PG product was the best-performing tested dose for supporting growth, nutrient utilization, antioxidant-related indices, and intestinal barrier-related indices in weaned piglets, and these responses were associated with changes in gut microbiota-related glutathione metabolism.

PMID:
42873456
Bibliographic data and abstract were imported from PubMed on 15 Oct 2026.

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