Local control of Cre-regulated genetic modeling in vivo using wireless implantable optoelectronics.

Authors

Trisha Bansal, Min-Kyu Lee, Courtney Coleman, Young-Mee Kim, Dane Hintermueller, Yaner Chen, Varsha Sreekanth, Chad R Haney, Olga A Karginova, Raudel Avila, Jacob Matsche, Shaluah Vijeth, Farah Yousef, Kento Yamagishi, Jiheon Kang, Kaitlyn Mowry, Minsung Kim, Sophianne Loh, Sachin Clark, Huong Huynh, Youngdo Kim, Kaiqing Zhang, Haohui Zhang, Shupeng Li, Yuanting Wei, Steve Seung-Young Lee, Yuru Liu, Anthony R Banks, Odile David, Peter T Toth, Yonggang Huang, Ameen Salahudeen, Cameron H Good, Jalees Rehman, John A Rogers, Andrei V Karginov

Published in

Science advances. Volume 12. Issue 42. Pages eaed1014. Oct 16, 2026. Epub Oct 14, 2026.

Abstract

Effective modeling of chronic human diseases in vivo requires precise spatiotemporal regulation of molecular processes at defined tissue locations. Optogenetic and optoelectronic tools can provide such control but targeting internal organs such as the lungs remain challenging. To address this, we developed E-LightR-Cre recombinase, enabling precise, spatially restricted gene activation with no dark-state activity and robust blue light responsiveness. To achieve local activation of E-LightR-Cre in specific organ sites, we engineered wireless, fully implantable optoelectronic devices enabling focal illumination of murine lungs with no discernible organ damage. The size of the activated area is controlled by modulating light intensity and duration. Using implanted optoelectronic devices, we activated E-LightR-Cre in a selected area of mouse lungs and achieved local regulation of targeted genes. Light-regulated expression of the oncogenic KRas-G12D mutant in mouse lungs induced local formation of oncogenic lesions. These results show that our protein engineering and adaptable optoelectronic approaches enable facile application of optogenetic tools for locally controlled disease modeling in vivo.

PMID:
42873865
Bibliographic data and abstract were imported from PubMed on 15 Oct 2026.

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