Monocyte-Derived Dendritic Cell Activation Is Broadly Conserved Across Immune Phenotype-Specific Ovarian Tumor Lysate Pools, With Donor-Associated Variation in Response Magnitude.

Authors

Emilija Paberalė, Yasmine Hassan Mohamed Rezk, Jan Aleksander Kraśko, Karolina Suveizdė, Vita Pašukonienė, Agata Mlynska

Published in

Journal of leukocyte biology. Oct 16, 2026. Epub Oct 16, 2026.

Abstract

Whether ex vivo monocyte-derived dendritic cell responses are shaped more by tumor immune context or donor-associated variation remains unclear. Monocyte-derived dendritic cells from nine healthy female donors were exposed for 8 h to three lysate pools generated from 27 high-grade serous ovarian tumors classified as immune-desert, immune-excluded, or immune-inflamed. Individual lysates showed phenotype-associated chemokine differences. The three pooled stimulation conditions elicited broadly conserved dendritic cell maturation responses and shared inflammatory transcriptional programs, with limited gene- and pathway-level differences. Permutational multivariate analysis attributed ≈58% of global transcriptional variance to donor identity (P = 0.001), whereas pooled lysate condition accounted for ≈4%, which was not significant (P = 0.756). Response magnitude varied continuously across donors and showed exploratory associations with baseline pathway activity in immature dendritic cells. Under the tested conditions, early monocyte-derived dendritic cell activation was broadly shared across the three lysate pools, while the transcriptional response magnitude was more strongly associated with donor identity.

PMID:
42885837
Bibliographic data and abstract were imported from PubMed on 16 Oct 2026.

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