Design and Analysis Strategies for Pooling in High Throughput Screening: Application to the Search for a New Anti-Microbial.

Authors

Byran J Smucker, Benjamin Brennan, Emily Rego, Meng Wu, Zhihong Lin, Brian M M Ahmer, Blake R Peterson

Published in

Statistics in medicine. Volume 45. Issue 23-24. Pages e70778.

Abstract

A major public health issue is the growing resistance of bacteria to antibiotics. An important part of the needed response is the discovery and development of new antimicrobial strategies. Require the screening of potential new drugs, typically accomplished using high-throughput screening (HTS). Traditionally, HTS is performed by examining one compound per well, but a more efficient strategy pools multiple compounds per well. In this work, we study several recently proposed pooling construction methods, as well as a variety of pooled high-throughput screening analysis methods, in order to provide guidance to practitioners on which methods to use. This is done in the context of an application of the methods to the search for new drugs to combat bacterial infection. We discuss both an extensive pilot study as well as a small screening campaign, and highlight both the successes and challenges of the pooling approach.

PMID:
42885925
Bibliographic data and abstract were imported from PubMed on 16 Oct 2026.

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