Protocol for the isolation, enrichment, and culture of murine adipose progenitor cells.

Authors

Ghazaleh Dadashizadeh, Fadi Khalaf, Stephanie Wojtowicz-Piotrowski, Marc G Jeschke

Published in

STAR protocols. Volume 7. Issue 4. Pages 104901. Oct 16, 2026. Epub Oct 16, 2026.

Abstract

We present a protocol for isolating and culturing murine adipose progenitor cells (APCs) from adipose tissue. We describe steps for tissue dissection, unit-normalized enzymatic digestion, stromal vascular fraction (SVF) isolation, APC enrichment, and cell culture. We then detail quality control procedures that maximize cell yield, viability, and purity. This protocol combines enzymatic SVF isolation with magnetic-activated cell sorting enrichment to obtain a highly enriched APC population. It emphasizes reproducibility through unit-normalized digestion, controlled processing conditions, and standardized quality control metrics.

PMID:
42886560
Bibliographic data and abstract were imported from PubMed on 17 Oct 2026.

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