Authors
David Lindgren, Rebecca Rosberg, Karolina I Smolag, Elinn Johansson, Sebastian Braun, Jonas Sjölund, Dimitra Manou, Enrico Pracucci, Bengt Phung, Katja Harbst, Pauline Jeannot, Yu Ching So, Paulina Bolivar, Maria Malmberg, Crister Ceberg, Johan Bengzon, Henrik Ahlenius, Göran B Jönsson, Kristian Pietras, Alexander Pietras
Published in
Cell reports. Volume 45. Issue 10. Pages 118119. Oct 15, 2026. Epub Oct 15, 2026.
Abstract
Glioblastomas initially respond to radiotherapy but invariably recur, often within high-dose radiation treatment fields. Although stromal radiation responses are incompletely understood, evidence suggests that the tumor microenvironment becomes tumor-supportive after therapy. Using a genetically engineered glioblastoma mouse model, we profiled healthy brain, primary tumors, and post-radiotherapy recurrences with single-cell and spatial transcriptomics and immunofluorescence. Across 13 non-neoplastic cell types and 10 tumor cell states, we mapped transcriptional adaptations accompanying progression from healthy brain to primary and recurrent glioblastoma. We find distinct astrocyte states linked to disease stage, including reactive, non-neoplastic astrocytes and tumor cells adopting reactive astrocyte-like phenotypes. Reactive astrocyte-like tumor cells with mesenchymal and injury-response signatures are enriched after radiotherapy and persist in recurrent tumors. Receptor-ligand interactions between reactive astrocytes and tumor cells include known and putative drivers of aggressiveness. These findings highlight convergent reactive astrocyte programs in astrocytes and tumor cells as potential mediators of glioblastoma radioresistance.
PMID:
42886556
Bibliographic data and abstract were imported from PubMed on 17 Oct 2026.
Read full publication at:
Please sign in
to see all details.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 3
- Comments 0