An inverse-protein-folding pre-trained model for predicting the mutational effects in protein-protein interactions.

Authors

Sihong Mao, Siwei Meng, Ye Xiang

Published in

iScience. Volume 29. Issue 10. Pages 117693. Oct 16, 2026. Epub Oct 06, 2026.

Abstract

Protein-protein interactions (PPIs) are fundamental to vast biological processes. Accurate prediction of mutation-induced changes in PPIs is crucial for protein engineering and drug discovery. Most existing PPI prediction approaches rely on refined physicochemical features or computationally intensive structural sampling. Here, we present delta-G message-passing-neural-network (DGMPNN), a message-passing neural-network framework for predicting the effects of mutations on PPIs. DGMPNN is pre-trained on an inverse protein folding (IPF) task and employs an adaptive residue-level attention pooling mechanism to enable effective transfer learning for predicting mutation-induced changes in protein binding affinity. The model achieves state-of-the-art performance on multiple benchmark datasets and deep mutational scanning (DMS) datasets. Experimental validation using computationally selected multi-site variants of the SARS-CoV-2 RBD-hACE2 complex showed strong agreement between predicted and experimentally measured binding affinities.

PMID:
42892932
Bibliographic data and abstract were imported from PubMed on 18 Oct 2026.

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