Authors
Wentao Li, Min Liu, Zhanyi Qu, Qianqian Deng, Ruijiang Liu, Zhan-Ao Wu
Published in
Bioelectrochemistry (Amsterdam, Netherlands). Pages 109473. Oct 19, 2026. Epub Oct 19, 2026.
Abstract
Telomerase reverse transcriptase promoter C228T mutations (TERTC228T) are strongly associated with poor outcomes in papillary thyroid cancer, necessitating sensitive detection methods. In this work, a "Signal-off" type of label-free and magnetically induced self-assembly electrochemical biosensors based on α-Fe2O3/Fe3O4@Au magnetic nanocomposites (MNCs) were developed for TERTC228T detection. Thiolated aptamers (Apts) for specific recognition of TERTC228T were immobilized on α-Fe2O3/Fe3O4@Au MNCs via AuS bonds, and bovine serum albumins (BSA) were employed to block non-specific sites and form α-Fe2O3/Fe3O4@Au-Apt/BSA biosensors. TERTC228T specifically hybridized with Apts, forming a rigid dsDNA film on the electrode. Enhanced steric hindrance and electrostatic repulsion suppressed electron transfer of [Fe(CN)6]3-/4-, leading to decreased current signals. The biosensors were evaluated by cyclic voltammetry, electrochemical impedance spectroscopy, and differential pulse voltammetry, the results demonstrated that the biosensors exhibited a wide linear range (102 pM- 106 pM), high sensitivity (LOD of 0.67 pM), and excellent selectivity, along with high reproducibility (RSD = 1.84%) and long-term stability (signal retention above 95% within 10 days), and enabled reliable detection in diluted human serum (recoveries of 98.1%-107.3%). Overall, the electrochemical biosensors offer a simple, rapid, and ultrasensitive platform for TERTC228T detection, with promising potential for molecular diagnosis and thyroid cancer risk stratification.
PMID:
42893892
Bibliographic data and abstract were imported from PubMed on 19 Oct 2026.
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