Role and mechanism of ATF6-mediated endoplasmic reticulum stress in ITLN-1 against cerebral ischemia-reperfusion injury.

Authors

Yanqing Liu, Lu Xu, Zhe Zhang, Caiqin Zhang, Peijie Liu, Pan Li, Shuya Wang, Ye Tian, Yang Yang, Xiaochen Niu

Published in

Life sciences. Pages 124746. Oct 19, 2026. Epub Oct 19, 2026.

Abstract

Intelectin-1 (ITLN-1), a novel adipocytokine, exerts neuroprotective effects against cerebral ischemia-reperfusion injury (CIRI), yet its molecular mechanisms remain unclear. This study focused on activating transcription factor 6 (ATF6), a key endoplasmic reticulum stress sensor, to explore its role in CIRI-induced neuronal injury and determine whether ITLN-1 mediates neuroprotection through ATF6 regulation.
We established an N2a cell hypoxia/reoxygenation (H/R) model and a mouse middle cerebral artery occlusion (MCAO) model. Using protein molecular docking, situ proximity ligation assay, Western blotting, mitochondrial membrane potential assays, and TUNEL staining, we investigated ATF6 activation in neuronal apoptosis and its upstream regulatory mechanisms.
H/R injury markedly induced ATF6 activation in N2a cells, triggering mitochondrial dysfunction and promoting apoptosis. ATF6 overexpression further aggravated these effects, confirming its critical pathogenic role in CIRI. Mechanistic analyses revealed that ITLN-1 physically associates with ATF6. Both ITLN-1 overexpression and exogenous recombinant ITLN-1 administration significantly suppressed excessive ATF6 activation, modulated the JNK signaling pathway, improved cell viability, reduced intracellular calcium overload and reactive oxygen species accumulation, and attenuated neuronal apoptosis. In vivo, ITLN-1 ameliorated neurological deficits, reduced neuronal damage, and alleviated demyelination, whereas ITLN-1 knockdown produced opposite effects.
Excessive ATF6 activation underlies CIRI-induced neuronal injury. ITLN-1 exerts neuroprotection by inhibiting ATF6 and modulating JNK signaling, thereby alleviating mitochondrial dysfunction and reducing apoptosis. These findings highlight the ITLN-1/ATF6 axis as a promising therapeutic target for ischemic stroke.

PMID:
42896704
Bibliographic data and abstract were imported from PubMed on 20 Oct 2026.

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