Salt-dependent osmoadaptation and metabolic responses of Lactiplantibacillus plantarum BS3-1 in fermented bamboo shoots.

Authors

Lili Xie, Xuyi Zhang, Kaiwei Shen, Xueli Wang, Shaoqi Zhou

Published in

Food microbiology. Volume 142. Pages 105316. Epub Sep 26, 2026.

Abstract

Salt concentration shapes the growth and metabolism of lactic acid bacteria in vegetable fermentations, yet the effects of salt gradients on the fermentation performance of suansun-derived Lactiplantibacillus plantarum BS3-1, together with associated temporal transcriptional changes in BS3-1 and metabolite variation in the fermentation broth, remain insufficiently characterized. Here, BS3-1 was evaluated across a 0-7% (w/w) salt gradient during suansun fermentation. The 0%, 3%, and 5% treatments were selected for time-series transcriptomic analysis of fermentation-derived cell pellets and untargeted metabolomic analysis of fermentation-broth supernatants. The 3% treatment yielded the highest sensory score (79.0/100 versus 72.2/100 in the no-added-salt treatment). On day 7, total acidity was 8.16 g/L at 1% salt and 3.60 g/L at 7%, and viable cells at 7% fell below the detection limit. BS3-1 transcriptional profiles and fermentation-broth metabolite compositions differed among salt treatments. In the 3% versus 0% comparison, 119 differentially expressed genes were shared across four time points, while an average of 598 differential metabolites was identified per time point. WGCNA identified 17 co-expression modules with significant module-trait associations. Association analysis integrating 126 candidate hub genes from four phenotype-associated modules with 30 shared differential metabolites retained 1100 gene-metabolite associations (|r| ≥ 0.65, global BH-FDR < 0.05). Overall, under the tested conditions, 3% salt provided the most favorable balance among viable-cell persistence, acidification, and sensory quality, while multi-omics analysis revealed covariation among BS3-1 transcriptional responses, fermentation traits, and fermentation-broth metabolite variation.

PMID:
42901669
Bibliographic data and abstract were imported from PubMed on 21 Oct 2026.

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