Plant cytokinin ortho-topolin riboside suppresses the proliferation of multiple myeloma cells by promoting β-catenin degradation.

Authors

Minji Kim, Wonku Kang, Sangtaek Oh

Published in

BMB reports. Oct 21, 2026. Epub Oct 21, 2026.

Abstract

Multiple myeloma involves the aberrant activation of the Wnt/β-catenin signaling pathway, resulting in β-catenin accumulation. Targeting this pathway presents a promising therapeutic approach for multiple myeloma. A cell-based chemical assay revealed that ortho-topolin riboside (oTR), a cytokinin naturally produced by Populus robusta, effectively suppressed the activation of β-catenin response transcription induced by Wnt3aconditioned medium. The mechanism underlying this suppression involved oTR-triggered phosphorylation of β-catenin at Ser33, Ser37, and Thr41, leading to its proteasomal degradation and a reduction in intracellular β-catenin levels. Furthermore, oTR consistently decreased β-catenin levels and inhibited the expression of Axin2, cyclin D1, and c-Myc-genes regulated by β-catenin/T cell factor signaling in multiple myeloma cells. Additionally, oTR inhibited the proliferation of multiple myeloma cells and promoted apoptosis, as evidenced by an increase in Annexin V-FITC-positive cells and elevated caspase-3/7 activity. These findings suggest that oTR exerts its antiproliferative effects by attenuating the Wnt/β-catenin pathway, indicating its potential as a therapeutic agent for preventing and treating multiple myeloma.

PMID:
42902569
Bibliographic data and abstract were imported from PubMed on 21 Oct 2026.

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