Magnoflorine Ameliorates Metabolic Dysfunction-Associated Steatotic Liver Disease by Targeting the NDUFV1 Subunit of Complex I to Regulate the ROS/AMPK Signaling Pathway.

Authors

Jiali Rao, Xuefei Li, Yushuang Zhang, Juan Guo, Baomin Feng, Yujie Lu, Xiaoyao Ma

Published in

Journal of agricultural and food chemistry. Volume 74. Issue 41. Pages 32318-32332. Oct 21, 2026.

Abstract

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a prevalent metabolic disorder with limited therapeutics. Magnoflorine (MAG), a natural alkaloid from edible plants, ameliorates MASLD; however, its direct targets remain unclear. Here, by integrating thermal proteome profiling and disease target databases, we identified NADH ubiquinone oxidoreductase core subunit V1 (NDUFV1), a core subunit of mitochondrial Complex I, as a key target of MAG. MAG directly binds NDUFV1, suppresses mitochondrial electron transport chain activity, and alleviates lipid-induced reactive oxygen species (ROS) accumulation. In high-fat cell models and MASLD mice, MAG contributes to the restoration of AMP-activated protein kinase (AMPK) signaling, promoting fatty acid β-oxidation and inhibiting lipid biosynthesis to maintain lipid homeostasis. Notably, the lipid-lowering effects of MAG were significantly attenuated in NDUFV1-knockdown cells. This study reveals that MAG regulates lipid metabolism by targeting NDUFV1, highlighting its potential as a natural bioactive compound for MASLD.

PMID:
42904973
Bibliographic data and abstract were imported from PubMed on 23 Oct 2026.

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