H₂O₂-driven dual-functional modification of Tremella fuciformis polysaccharides: Molecular weight reduction and oxidative group engineering for multidimensional bioactivity control.

Authors

Fei Lin, Zhibin Wu, Fang Luo, Zhenyu Lin, Jian Wang, Wu Wang, Rui Li, Guanhua Fang, Bin Qiu

Published in

International journal of biological macromolecules. Volume 331. Issue Pt 2. Pages 148513. Epub Oct 24, 2025.

Abstract

High molecular weight and inert functional groups limit the biomedical utility of natural polysaccharides, such as Tremella fuciformis polysaccharides (TFPs). In this study, we describe a H₂O₂-driven, one-step method for the concurrent oxidation and degradation of TFPs, which reduces the molecular weight from 1079.6 to 4.6 kDa while introducing aldehyde (0 %-0.99 %) and carboxyl (1.22 %-9.80 %) groups. By altering the H₂O₂ concentration, we obtained oxidized TFPs (OTFPs) with defined properties, namely OTFP-1 (13.5 kDa, 0.36 % aldehyde) exhibiting low cytotoxicity (73 % viability at 15 mg/mL); OTFP-3 (5.0 kDa, 0.80 % aldehyde) exhibiting enhanced antioxidant (DPPH/ABTS: 83 %/89 %), anti-inflammatory (IL-6/TNF-α reduction: 46 %/53 %), and ultraviolet B repair (36 % recovery) activities; and OTFP-4 (4.6 kDa, 0.99 % aldehyde) displaying broad-spectrum antibacterial activity (minimum inhibitory concentration: 7-9 mg/mL). The aldehyde groups also yielded injectable, self-healing hydrogels through dynamic Schiff base bonds. This green strategy decouples molecular weight and functionalization and provides a versatile platform for tailored bioactive materials.

PMID:
42904780
Bibliographic data and abstract were imported from PubMed on 23 Oct 2026.

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