Authors
Maurice Mürköster, Mateusz Balka, Christina J Kamp, Judit Källström, Linus Karlsson, Claudia A Rodriguez Aparicio, Erik Tunäng, Hanna-Louise Ögren, Silvia Theopold, Alister J Cumming, Alex Toftgaard Nielsen, Daniel O Daley
Published in
Frontiers in bioengineering and biotechnology. Volume 14. Pages 1956994. Epub Oct 07, 2026.
Abstract
Recombinant protein production can be a challenging process. To help users troubleshoot and optimize experiments in Escherichia coli we have compiled and characterized a toolbox of biosensor plasmids. It contains a biosensor that monitors the translational efficiency of the recombinant protein and quality control biosensors that detect unwanted events, such as protein misfolding in the cytoplasm, inefficient translocation through the Sec translocon, or protein misfolding in the periplasm. The biosensors were combined with different fluorescent proteins so that multiple events could be measured in parallel, in the same cell. The toolbox is a resource for diagnosing protein production problems and evaluating solutions that mitigate these problems.
PMID:
42909106
Bibliographic data and abstract were imported from PubMed on 23 Oct 2026.
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