Chloroquine inhibits Spring viremia of carp virus replication by modulating autophagy and metabolic pathways.

Authors

Yuchao Zhu, Shuyang Chen, Yue Wang, Jian Shen, Libo He, Yaping Wang, Dong Wang, Shuyan Miao, Guobin Chang, Pengfei Chu

Published in

Frontiers in immunology. Volume 17. Pages 1920055. Epub Oct 07, 2026.

Abstract

Spring viremia of carp virus (SVCV) severely threatens cyprinid aquaculture, yet the role of autophagy in SVCV infection remains unclear. Here, SVCV infection was associated with enhanced autophagic activity and autophagic flux in common carp and EPC cells. Pharmacological perturbation of autophagy revealed that rapamycin treatment increased SVCV gene expression and aggravated SVCV-induced cytopathic effects, whereas chloroquine blocked autophagic flux, suppressed viral gene expression, and mitigated virus-induced cellular damage. Transcriptomic analysis identified 1,868 differentially expressed genes after chloroquine treatment, with major changes in lysosome/autophagy-related pathways, glycan degradation, ion transport, cell cycle, DNA replication, and DNA repair. Metabolomic and integrated multi-omics analyses revealed changes in amino sugar and nucleotide sugar metabolism, steroid biosynthesis, riboflavin metabolism, and amino acid metabolism. Functional validation showed that diflorasone and all-trans-retinal reduced SVCV-G expression, alleviated cytopathic effects, and improved survival after SVCV infection. These findings suggest that SVCV infection is associated with enhanced autophagic flux, whereas chloroquine treatment blocks autophagic flux and is accompanied by reduced SVCV gene expression.

PMID:
42909045
Bibliographic data and abstract were imported from PubMed on 23 Oct 2026.

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