Photostable rhodamine 6G-based fluorescent probe for selective and sensitive visualization of dynamic lysosomal pH.

Authors

Dongyan Hu, Jiao Guan, Jiaming Luo, Liushuang Zhang, Zhijuan Bao

Published in

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. Volume 367. Issue Pt 2. Pages 128994. Oct 16, 2026. Epub Oct 16, 2026.

Abstract

Lysosomal pH is tightly linked to diverse cellular physiological and pathological processes, and real-time monitoring of its dynamic shifts is critical for uncovering underlying disease mechanisms. Herein, we developed a new lysosome-targeted pH fluorescent probe RGB with facile synthesis, low cytotoxicity, favorable biocompatibility and efficient cell membrane permeability. Its fluorescence emission intensity exhibits a well-defined linear correlation with environmental pH, enabling rapid, sensitive and quantitative detection of subtle pH changes at the single-lysosome level. RGB functions via a pH-triggered spirolactam ring-opening ICT fluorescence turn-on mechanism, with a measured pKa value of 4.12. Confocal fluorescence imaging results verify that RGB can evaluate oxidative stress-induced cellular damage and dynamically visualize autophagy in live tumor cells. As a robust molecular imaging reagent, RGB holds great promise for subcellular pH detection and basic mechanistic exploration of lysosome-associated disorders.

PMID:
42913037
Bibliographic data and abstract were imported from PubMed on 24 Oct 2026.

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