Construction of a Golgi apparatus-localization polarity-responsive fluorescent probe with large Stokes shift to screen the small-molecule drugs for the inhibition of ferroptosis.

Authors

Xu Yang, Wenqing Han, Qi-Yun Feng, Baoli Dong, Tao Yue

Published in

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. Volume 367. Issue Pt 2. Pages 128990. Oct 16, 2026. Epub Oct 16, 2026.

Abstract

Ferroptosis is a distinctive cell death form and has close connections with many diseases, and screening of ferroptosis-associated drug has great importance to delve into creative approaches against the currently intractable diseases. Herein, we developed a method for the ferroptosis-related small-molecule drugs screening by a GA-targeting polarity-responsive fluorescent probe (GP-1). The probe GP-1 showed large Stokes shifts in solvents (up to 194 nm), and displayed desirable sensitivity and selectivity to polarity. GP-1 was primarily distributed in Golgi apparatus (GA) rather than the other common organelles such as lysosome, mitochondria and endoplasmic reticulum (ER). Fluorescence imaging experiments revealed that erastin-induced ferroptosis could raise GA polarity noticeably. By using GA polarity as an indicator to assess the bioactivity of various small-molecule drugs, the imaging data showed that the natural active molecules (e.g. baicalein, rutin, curcumin, DHLA, lipoic acid, retinol, VE, 7-DHC) and edaravone can inhibit the significant changes of GA polarity, while the common Fe(II)-complexing agents (citric acid, tartaric acid) displayed nearly no inhibition effects to ferroptosis.

PMID:
42913027
Bibliographic data and abstract were imported from PubMed on 24 Oct 2026.

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