Authors
Vanessa de Cássia Martins Pires Ferreira, Victor Hugo Carvalho, Patrícia Aline Boer, Luís Fernando Barbisan
Published in
Environmental and molecular mutagenesis. Volume 67. Issue 8-9.
Abstract
This study evaluated the susceptibility to lung and kidney carcinogenesis induced by N-bis(2-hydroxypropyl)nitrosamine (DHPN) in female rats whose dams received a low-protein diet (LPD) during gestation. Pregnant Wistar rats were fed normal protein diet (NPD, 17% protein) or LPD (6% protein). After parturition, all dams were fed NPD until weaning. Then, female offspring were distributed into four groups (NPD, LPD, NPD + DHPN, and LPD + DHPN) and fed NPD. At 6 weeks old, two groups received 0.2% DHPN in drinking water for 4 weeks, and some females were euthanized 24 h after carcinogen stopping, while others were euthanized at Week 26. First, the lung was removed, fixed, histologically processed, and tissue sections were immunohistochemically stained for γ-H2AX. Second, the lung and kidneys were removed, fixed, histologically processed, and hematoxylin-eosin stained tissue sections were obtained for histopathological analysis. Female rats whose dams received LPD developed a higher mean number of pulmonary epithelial cells positive for γ-H2AX (p < 0.005) and bronchioloalveolar hyperplasia and adenomas (p < 0.001 and p < 0.001) in the lung compared with their counterparts. LPD did not alter the development of atypical tubule hyperplasia in the kidney. These findings indicated that LPD increased DHPN-induced lung carcinogenesis in female rats.
PMID:
42920348
Bibliographic data and abstract were imported from PubMed on 24 Oct 2026.
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