Generation of Murine Chimeric Antigen Receptor T Cells for Adoptive T Cell Therapy for Melanoma.

Authors

Amorette Barber

Published in

Methods in molecular biology (Clifton, N.J.). Volume 3089. Pages 591-601.

Abstract

Expression of chimeric antigen receptors enables T cells to be redirected toward melanoma-associated antigens in an MHC-independent manner. Retroviral transduction can be used to introduce these receptor constructs into murine CD4⁺ and CD8⁺ T cells. Here, we outline the generation of retroviral supernatants and the activation, transduction, expansion, and selection of murine T cells expressing the chimeric-PD1 receptor. This protocol can be adapted for any murine chimeric antigen receptor construct.

PMID:
42926298
Bibliographic data and abstract were imported from PubMed on 25 Oct 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.