Assessing the Antitumor Immune Effects of Gut Microbiota in a Preclinical Melanoma Model.

Authors

Parastoo Sabaeifard, Wenling Li, Priscilla Del Valle, Rebecca Suet-Yan Chiu, Jana Dakour, Nicole Poulides, Shuheng Gan, Jake N Lichterman, Christina M Zarek, Xiaowei Zhan, Andrew Y Koh

Published in

Methods in molecular biology (Clifton, N.J.). Volume 3089. Pages 551-590.

Abstract

Antitumor immune responses are regulated by a multitude of factors, including the gut microbiota. Specific bacterial taxa and metabolites have been correlated with improved responses to immune checkpoint inhibitor therapy in melanoma patients. Gut microbiota and microbiota-derived metabolites can modulate antitumor immune responses by increasing antigen presentation capabilities and enhancing cytotoxic T-cell effector functions. To assess the effects of gut microbiota on antitumor immune responses, we optimized the following protocols focused on immune checkpoint inhibitor therapy in a preclinical melanoma model. Analysis of tumor-infiltrating and circulating lymphocytes, bacterial translocation and growth, and immune cell activation provides a foundation for microbiota/cancer immunology research.

PMID:
42926297
Bibliographic data and abstract were imported from PubMed on 25 Oct 2026.

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