Characterization of an Anti-Tryptophan 2,3-Dioxygenase Monoclonal Antibody and Use in a Melanoma Model.

Authors

Summer Sedano, Kaylend Manley, George C Prendergast, Marie R Webster, Lisa D Laury-Kleintop

Published in

Methods in molecular biology (Clifton, N.J.). Volume 3089. Pages 501-516.

Abstract

Monoclonal antibodies have served as research tools to characterize specific proteins and biomolecules to understand their role in biological processes and disease. However, because of nonspecific reactivity with other components in samples, specific reactivity can be difficult to interpret. Using web-based tools, standard hybridoma technology, and standard immunostaining methods, we describe the development of a tryptophan 2,3-dioxygenase 2 (TDO2)-targeted antibody with high specificity. In addition to providing a unique reagent needed as a research tool to investigate the role of TDO2 in preclinical models and human tissue samples, herein we provide methods to characterize TDO2 expression in human tissue samples and murine disease models. We note also that the general protocol described in this chapter will be useful to investigators interested in developing monoclonal antibodies against other targets of interest as well.

PMID:
42926293
Bibliographic data and abstract were imported from PubMed on 25 Oct 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.