Discovery of Salivary Metabolomic Biomarkers for Canine Oral Melanoma and Squamous Cell Carcinoma via CIL LC-MS Analysis.

Authors

Sekkarin Ploypetch, Xian Luo, Shuang Zhao, Gunnaporn Suriyaphol, Liang Li

Published in

Methods in molecular biology (Clifton, N.J.). Volume 3089. Pages 417-425.

Abstract

In this case-control cross-sectional study, we examined the diagnostic potential of salivary metabolomics to differentiate canine oral melanoma (OM) and oral squamous cell carcinoma (OSCC) from benign oral tumors (BN). Using high-performance chemical isotope labeling liquid chromatography-mass spectrometry (CIL LC-MS) targeting at the amine/phenol submetabolome, we analyzed saliva from 65 dogs across malignant, benign, and healthy groups. Our results revealed distinct metabolic clustering among the groups, identifying 154 significantly altered metabolites in OM and 66 in OSCC compared to BN (P_adj < 0.05). Notable biomarkers included elevated tryptophyl-threonine and decreased 1,2-dihydroxynaphthalene-6-sulfonic acid in OM, while OSCC was characterized by reduced seryl-arginine and sarcosine. These findings, and the protocol described herein that was used to obtain them, demonstrate the broad coverage of the salivary submetabolome and emphasize the potential of noninvasive metabolic profiling as a practical, high-throughput approach for accurate diagnosis of canine oral cancers in clinical settings.

PMID:
42926288
Bibliographic data and abstract were imported from PubMed on 25 Oct 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.