Integrated Functional and Metabolomic Profiling of Melanoma Cells Treated with Redox-Active Compounds.

Authors

Marcella Bonanomi, Daniela Gaglio

Published in

Methods in molecular biology (Clifton, N.J.). Volume 3089. Pages 389-402.

Abstract

Metabolic plasticity is a hallmark of melanoma progression and therapy resistance. This chapter describes a reproducible protocol for comprehensive metabolic profiling of melanoma cells exposed to redox-active compounds such as emodin and aloe-emodin. The method integrates cell proliferation assays, mitochondrial functional analysis using Seahorse extracellular flux measurements, reactive oxygen species (ROS) quantification, LC-MS-based metabolomics, stable-isotope tracing, and ¹³C metabolic flux analysis (¹³C-MFA). The workflow enables quantitative characterization of treatment-induced alterations in energy metabolism, redox balance, and central carbon fluxes in melanoma cell lines with distinct metabolic phenotypes. The procedures are presented in a step-by-step format suitable for researchers unfamiliar with the individual techniques and can be adapted to other cancer models or metabolic perturbations.

PMID:
42926286
Bibliographic data and abstract were imported from PubMed on 25 Oct 2026.

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