Preparation of Melanoma Tissue for Multiplex Immunofluorescence Histology Analysis of Immune Infiltration and Spatial Organization.

Authors

Victoria Ann Remley, Marya Dunlap-Brown, Kelly T Smith, Sheri Vanhoose, Sara J Adair, Todd W Bauer, Ileana S Mauldin

Published in

Methods in molecular biology (Clifton, N.J.). Volume 3089. Pages 349-361.

Abstract

Immunotherapies extend survival in melanoma patients by increasing the immune system's capacity to detect tumors and mediate tumor cell death. Understanding mechanisms of antitumor immunity requires evaluating changes in immune infiltrate composition, activation, and spatial distribution within the tumor microenvironment (TME). Despite this need, relatively few methods allow for detailed analysis of both the structural organization and immunological characteristics of tumor-infiltrating immune cells in preclinical cancer models. In this chapter, we present a multiplex immunofluorescence staining approach for identifying, characterizing, and quantifying tumor-infiltrating immune cells in murine melanoma, allowing evaluation of how therapies influence immune infiltration in preclinical models.

PMID:
42926283
Bibliographic data and abstract were imported from PubMed on 25 Oct 2026.

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