Guidelines and Best Practices for O-GlcNAcylation, OGT, and OGA Imaging in Fixed Cells and Tissues.

Authors

Bruno C Rodrigues, Amulya Rao, Chad Slawson

Published in

Glycobiology. Oct 26, 2026. Epub Oct 26, 2026.

Abstract

O-GlcNAcylation is a nutrient-sensitive post-translational modification, dynamically cycled by the opposing enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). As its detection by any single reagent remains partial and uneven, the imaging literature on O-GlcNAc has yet to converge on a standardized approach to fixation chemistry, antibody choice, and image quantification, which challenges direct cross-study comparison. To navigate O-GlcNAc complexity for fixed-cell imaging, we provide an actionable, model-organism-specific methodological framework spanning sample fixation, permeabilization, blocking, detection chemistry, acquisition, and validation for the O-GlcNAc modification itself as well as for OGT and OGA. We further propose a minimal reporting checklist, aligned with current community standards for quantitative fluorescence microscopy, intended to let readers judge, reproduce, and build on O-GlcNAc imaging data.

PMID:
42932229
Bibliographic data and abstract were imported from PubMed on 27 Oct 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.