Low Input RNA Sequencing of Freshly Isolated Muscle Stem Cells.

Authors

Fayez Issa, Joana Esteves de Lima

Published in

Methods in molecular biology (Clifton, N.J.). Volume 3093. Pages 197-209.

Abstract

Bulk RNA sequencing allows for the detection and quantification of mRNA transcripts from pooled cell populations, tissue sections, and biopsies. However, working with rare populations with limited amount of input requires specialized handling to ensure high-quality data. Here, we describe a detailed, optimized low-input protocol for bulk RNA sequencing on FACS-isolated satellite cells from mouse hindlimb skeletal muscle tissues. This protocol covers sample preparation, library preparation, sequencing, and data analysis.

PMID:
42932063
Bibliographic data and abstract were imported from PubMed on 27 Oct 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.