A Highly Permissive K-C4 Cell Clone for Genotype II African Swine Fever Virus Reveals Passage-Dependent Hemadsorption Changes Associated with EP402R Deletion.

Authors

Eun-Gyeong Lee, Ju-Hun Kim, Sarah Lim, Sung-Hyun Moon, Hyunji Gu, Jun Young Park, Dae-Min Kim, Gun-Hee Lee, Ho-Seong Cho, Kyeong-Man Kim, Dongseob Tark

Published in

Veterinary sciences. Volume 13. Issue 10. Sep 28, 2026. Epub Sep 28, 2026.

Abstract

African swine fever virus (ASFV) research commonly relies on primary porcine alveolar macrophages (PAMs), whose limited availability and variability hinder standardized virus isolation and propagation. This study aimed to establish a continuous cell line highly susceptible to genotype II ASFV and characterize phenotypic and genomic changes during serial passage. Thirty COS-1-derived clones were screened after infection with Korea/Pig/PAJU1/2019 using quantitative real-time polymerase chain reaction, immunofluorescence, and hemadsorption assays. Selected clones underwent serial passage, and viruses from passages 5 and 10 in the most susceptible clone were analyzed by whole-genome sequencing. K-C4 supported stable ASFV replication, with a mean viral genome level of 8.80 ± 0.55 log₁₀ copies/mL, compared with 7.38 ± 0.90 log₁₀ copies/mL in PAMs. Productive infection was confirmed by p72-specific immunofluorescence. The parental and passage 1 viruses retained hemadsorption, whereas the passage 5 virus lacked detectable hemadsorption despite continued replication, and hemadsorption reappeared by passage 10. Whole-genome analysis identified passage-associated deletions of differing extent within EP402R, encoding the hemadsorption-associated CD2-like viral protein (CD2v), which corresponded with the hemadsorption phenotype at each passage. These findings establish K-C4 as a highly permissive platform for genotype II ASFV propagation and characterization while emphasizing the need to monitor phenotypic and genomic stability during serial passage.

PMID:
42944120
Bibliographic data and abstract were imported from PubMed on 27 Oct 2026.

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