Coumarin-Ferulic Acid Hybrid Mediates HT-29-Selective Growth Inhibition and Proteomic Profiling.

Authors

Sucheewin Krobthong, Meng Chieh Yang, Pattarawit Rukthong, Satsawat Visansirikul, Kittisak Sripha, Arnatchai Maiuthed

Published in

Molecules (Basel, Switzerland). Volume 31. Issue 20. Oct 17, 2026. Epub Oct 17, 2026.

Abstract

Coumarin-based molecular hybrids provide a framework for examining how structural changes influence cellular responses. This study compared four hybrids and three reference compounds across ten human cancer cell lines, with the lead hybrid also tested in non-cancerous Beas-2B cells, followed by functional assays and quantitative expression proteomics in HT-29 cells. Of 70 compound-cell-line combinations, the ferulic ester hybrid (FEC) was the only compound to yield a reproducibly measurable IC₅₀ within the primary screening range, reaching 42.8 ± 4.7 µM in HT-29 cells. Replacing the ferulic moiety or the ester linkage reduced activity within this series. HCT116 cells did not reach 50% inhibition at up to 100 µM. Basal PPARγ abundance did not consistently explain sensitivity across the panel. FEC reduced metabolic activity and colony formation, whereas annexin V/propidium iodide analysis showed a smaller apoptotic response. Proteomics identified coordinated decreases in DNA replication and translation proteins and a modest increase in endoplasmic reticulum protein-folding enzymes. These findings support a structure-dependent and cell-context-dependent antiproliferative response despite moderate potency.

PMID:
42943798
Bibliographic data and abstract were imported from PubMed on 27 Oct 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.