Protocol for the extraction and purification of shikimic acid from Escherichia coli fermentative media.

Authors

Pedro Silva, Ana C Afonso, Manuel Simões, Lúcia Santos, Margarida M S M Bastos

Published in

STAR protocols. Volume 7. Issue 4. Pages 104919. Oct 27, 2026. Epub Oct 27, 2026.

Abstract

Shikimic acid (SA) is a valuable pharmaceutical precursor used in drug synthesis. Here, we present a protocol for isolating and purifying SA from Escherichia coli cultures. We describe steps for combining cell disruption, centrifugation, filtration, preparative anion-exchange chromatography, elution, and lyophilization. We detail procedures for identifying SA-containing fractions by Fourier transform infrared (FTIR) spectroscopy, followed by ethanol-based recrystallization and FTIR characterization of the purified product. This protocol provides an integrated downstream processing strategy for microbial SA production.

PMID:
42945208
Bibliographic data and abstract were imported from PubMed on 28 Oct 2026.

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