Protocol for visualizing microtubules in transzonal projections of mouse ovarian follicles.

Authors

Takao Tsurumaki, Akihiro Aikawa, Masamitsu Sato, Mika Toya

Published in

STAR protocols. Volume 7. Issue 4. Pages 104924. Oct 28, 2026. Epub Oct 28, 2026.

Abstract

Visualizing microtubules within tissues remains technically challenging, limiting our understanding of their organization and function in vivo. Here, we present a protocol for visualizing microtubules in transzonal projections (TZPs), cellular projections that connect ovarian somatic cells and oocytes within mouse ovarian follicles. We describe steps and conditions for ovarian fixation, cryosection preparation, and immunostaining to preserve fine microtubule structures. We then detail procedures for super-resolution imaging to visualize fine microtubules within TZPs. For complete details on the use and execution of this protocol, please refer to Aikawa et al.¹.

PMID:
42949913
Bibliographic data and abstract were imported from PubMed on 29 Oct 2026.

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