Near-infrared excited core-shell upconversion composites for living cells proteome labeling.

Authors

Chen Le, Jiang Qianqian, Jia Jingjing, Jiang Bo, Xu Sai, Zhang Lihua, Zhang Yukui

Published in

Analytica chimica acta. Volume 1425. Pages 346307. Dec 01, 2026. Epub Sep 21, 2026.

Abstract

Near-infrared excitation effectively eliminates interference from intracellular visible-light-absorbing photosensitizers for living cells proteome labeling. Herein, a core-shell upconversion composites (CS-NaYF₄@MOF) were developed for NIR activated proteome proximity labeling in living cells. A NIR-responsive photosensitizer was fabricated by in situ growth of a Cu-TCPP-based metal-organic framework (MOF) shell on Nd³⁺-sensitized core-shell upconversion nanoparticles. Upon 808 nm NIR excitation, the composites generate green upconversion luminescence to efficiently activate Cu(II) meso-Tetra(4-carboxyphenyl)porphine (Cu-TCPP) for reactive oxygen species production, enabling protein labeling in living cells with nucleophilic substrate. The characterizations confirm the successful formation of a crystalline MOF coating with homogeneous elemental distribution. Cellular assays reveal low photo-toxicity, allowing time- and concentration-dependent protein labeling under NIR light irradiation. By combining click chemistry and mass spectrometry, high-throughput proteome identification in living cells is successfully realized. This work not only extends the application of MOF-based materials in proteomics, but also offers a NIR excited strategy for living cells proteome labeling.

PMID:
42951544
Bibliographic data and abstract were imported from PubMed on 29 Oct 2026.

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