A KIT-6 mesoporous silica nanoparticle-based chemiluminescent aptasensor for sensitive detection of 8-Oxo-dG.

Authors

Maryam Imani, Zahra Vaezi, Hossein Naderi-Manesh

Published in

Analytica chimica acta. Volume 1425. Pages 346279. Dec 01, 2026. Epub Sep 16, 2026.

Abstract

Excessive accumulation of reactive oxygen species disrupts repair mechanisms and causes irreversible damage to lipids, proteins, and DNA. A target-responsive chemiluminescence sensor was developed for the sensitive detection of 8-OxodG, an important biomarker of oxidative DNA damage.
In this chemiluminescence sensor, acridinium ester (AE) was loaded into KIT-6 mesoporous silica nanoparticles and capping the pores with an 8-oxodG-specific aptamer. In the presence of 8-oxodG, target-aptamer recognition induced aptamer detachment and AE release. The released AE reacted with H₂O₂ to generate a concentration-dependent chemiluminescence signal. Under optimized conditions, the sensor exhibited a response range of 0.01-100 μM and a detection limit of 4.8 nM in PBS, by achieving a satisfactory recovery rate of 70.4-108.9% with RSD of 12.7-20.0%. The sensor also showed good selectivity and was successfully evaluated in spiked artificial urine with detection limit of 22.03 nM.
The combination of aptamer-controlled molecular gating, the interconnected pore structure of KIT-6, and chemiluminescent signal generation provides a sensitive controlled-release platform for 8-oxodG detection and supports its further development for biological-sample analysis.

PMID:
42951527
Bibliographic data and abstract were imported from PubMed on 29 Oct 2026.

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