Authors
Pin Kai Liao, Puchong Kijamnajsuk, Shauqi Abdai Lubis, Agustin Krisna Wardani, Jyh Jian Chen
Published in
Analytical biochemistry. Pages 116284. Oct 29, 2026. Epub Oct 29, 2026.
Abstract
This study reports the development of an integrated, portable analytical platform combining a micro-spectrometer with loop-mediated isothermal amplification (LAMP) for the rapid, on-site detection of Vibrio vulnificus. The system, controlled by LinkIt 7697 microprocessors, achieves precise thermal regulation within ±0.32 °C at 64 °C and features a customized optical path for real-time fluorescence monitoring. Utilizing calcein as a metal-sensitive fluorescent indicator, the device captures the spectral shift and intensity enhancement resulting from the displacement of manganese ions during DNA synthesis. Unlike traditional photodiode-based sensors, the integrated micro-spectrometer enables full-spectrum analysis at 1-minute intervals, providing superior discrimination between true positive signals and background autofluorescence. Experimental results demonstrate successful detection of Vibrio vulnificus genomic fragments within 40 minutes, achieving a detection limit of 0.1 pg/μL, with data automatically synchronized to a cloud database for remote epidemiological surveillance. The combination of high-fidelity spectral acquisition and isothermal chemistry offers a robust, cost-effective solution for food safety monitoring and point-of-care testing in resource-limited environments.
PMID:
42956954
Bibliographic data and abstract were imported from PubMed on 30 Oct 2026.
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