Authors
Anita B Dabek, Anthony M Beaney, Mauran Mahendra, Mikayla Olin, Anastasia Eftychidou, Joanna D Kolacz, Natalia Papalamprou, Marissa R Dickson, Patrick Romann, Colin Jaques
Published in
Biotechnology progress. Oct 30, 2026. Epub Oct 30, 2026.
Abstract
Increasing protein therapeutics production by adopting intensified perfusion cultures places high demand on the mass transfer capabilities of single-use bioreactors (SUB). Here, we compare these capabilities for four SUB designs against worst-case O2 transfer requirements for supporting Chinese hamster ovary (CHO) perfusion cultures. Impact of power dissipation, gas flow rate, O2 enrichment, pCO2 and sparge-hole size on O2 and CO2 mass transfer (OTR and CTR) was characterized. Only one design delivered sufficient O2 and removed sufficient CO2 to support a worst-case cell line at 100 × 106 viable cells/mL with a single-sparger at both 50 and 1000 L scale. Larger-scale versions of 3 designs were also tested. Performance at a 50-L scale was not predictive of large-scale performance (200 or 1000 L) for any design. Changes in the volume and composition of the gas phase during residence in the vessels were characterized. Substantial changes were found. Calculations of predicted culture pCO2 were performed across the design space, assuming a respiratory quotient of 1. A response surface was constructed to predict culture pCO2 and used to examine the consequences of various strategies for increasing OTR. Most strategies disproportionately increase OTR relative to CTR, leading to an increase in culture pCO2. The implications on SUB design and scale-up for high-intensity perfusion cultures are discussed.
PMID:
42958888
Bibliographic data and abstract were imported from PubMed on 30 Oct 2026.
Read full publication at:
Please sign in
to see all details.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 5
- Comments 0