Polygon-synchronized line-confocal microscopy with offset-slit detection for high-speed volumetric fluorescence imaging.

Authors

Shuhao Shen, Tingxue Xin, Nanguang Chen, Xueli Chen

Published in

Optics letters. Volume 51. Issue 21. Pages 5922-5925. Nov 01, 2026. Epub Sep 28, 2026.

Abstract

Fast volumetric fluorescence imaging requires both optical sectioning and high acquisition speed. We present a polygon-synchronized line-confocal microscope (Poly-LSCM) that combines a 16-facet polygon scanner, direct sCMOS detection, and offset-slit image reconstruction. The system eliminates image stitching and enables high-speed line-confocal imaging with improved scan uniformity. A subtraction-based reconstruction strategy further suppresses out-of-focus background and enhances image contrast. Poly-LSCM achieves a lateral resolution of 510 nm, an axial full width at half maximum of 738 nm, and imaging rates exceeding 200 fps. We demonstrate volumetric imaging of thick Thy1-EGFP mouse brain tissue and high-speed imaging of fluorescent microspheres in microfluidic channels. These results establish Poly-LSCM as a practical approach for high-throughput fluorescence imaging with millisecond-scale temporal resolution.

PMID:
42962088
Bibliographic data and abstract were imported from PubMed on 31 Oct 2026.

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