Effects of Different Culture Conditions on the Content of N6-(2-hydroxyethyl)-Adenosine in Cordyceps chanhua (Ascomycota).

Authors

Fangping Sa, Guoyue Song, Yupeng Ge, Xiaohong Wang, Weihuan Li, Xian-Hao Cheng

Published in

International journal of medicinal mushrooms. Volume 28. Issue 10. Pages 19-34.

Abstract

Cordyceps chanhua is a significant natural source of N6-(2-hydroxyethyl)-adenosine (HEA), yet the regulatory mechanisms governing HEA biosynthesis remain poorly understood. In this study, 40 wild C. chanhua strains were isolated from samples collected in Zhejiang and Hubei provinces. Together with one laboratory-main- tained strain (CH1), a total of 41 strains were evaluated through fruiting assays for bioconversion efficiency and HEA production. All isolates were identified as the MAT1-2 mating type. Bioconversion efficiencies varied widely, ranging from 20% to 160%. Based on these results, strains CH1, CH21, and CH24 were selected for further investigation. The influence of naphthalene acetic acid (NAA) concentration, sodium chloride (NaCl) concentration, cultivation duration, and substrate composition and ratios on HEA accumulation were systematically assessed. Among single-substrate me- dia, mung beans supported the highest HEA levels in fruiting bodies, whereas soybean medium produced the greatest HEA content in mycelial sclerotia. When oats and mung beans were combined at a 20:10 ratio, HEA levels in fruiting bodies reached their maximum. Under NAA treatment, mycelial sclerotia demonstrated peak HEA content at 2 g/L, corresponding to a 3.17-fold increase relative to the control. With NaCl supplementation, the highest HEA concentra- tion in fruiting bodies was observed at 10 g/L, a 2.9-fold increase over the control. Moreover, HEA accumulation in fruiting bodies increased initially and then declined with extended cultivation, a pattern unaffected by NaCl treatment. These findings provide a laboratory-scale reference for optimizing cultivation parameters to enhance HEA production in C. chanhua.

PMID:
42962180
Bibliographic data and abstract were imported from PubMed on 31 Oct 2026.

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