Genome-wide association study integrated with transcriptomic validation reveals polygenic architecture of Vibrio parahaemolyticus resistance in hybrid abalone (Haliotis discus hannai ♀ × H. fulgens ♂).

Authors

Chao Fan, Hanling Gan, Zhen Zhao, Linyue Wang, Yanyu Yao, Lu Li, Yilei Wang, Ziping Zhang

Published in

Fish & shellfish immunology. Pages 111807. Oct 31, 2026. Epub Oct 31, 2026.

Abstract

Vibrio parahaemolyticus is a major bacterial pathogen threatening abalone aquaculture, yet the genetic basis of host resistance remains poorly understood. Here, we combined a controlled challenge test, super genotyping-by-sequencing (Super-GBS), genome-wide association study (GWAS), and RT-qPCR to investigate resistance to V. parahaemolyticus in DF(Green Pacific hybrid abalone, Haliotis discus hannai ♀ × H. fulgens ♂, DF)abalone. A challenge dose of 3 × 10⁷ CFU/mL was selected to discriminate resistant and susceptible individuals, and after final quality control, 478,959 SNPs were included in the GWAS, which identified 862 FDR-significant SNPs, including 332 that also exceeded the Bonferroni-corrected threshold. Candidate gene and enrichment analyses highlighted multiple processes related to immune recognition, signal transduction, lysosome function, and metabolic regulation. Several genes, including tlr2, prkg1, slc7a9, man2b1, and htr6, were further supported by differential expression between resistant and susceptible groups. Functionally, these genes may represent different components of the host response to bacterial infection, including pathogen recognition, immune signal transduction, amino acid transport, lysosomal degradation, and metabolic regulation. Their coordinated involvement suggests that resistance to V. parahaemolyticus is mediated by an integrated immune-response network rather than by a single gene or pathway. Overall, this study demonstrates that resistance to V. parahaemolyticus in DF abalone is a complex polygenic trait and provides valuable genomic resources and candidate markers for the development of disease-resistant breeding strategies.

PMID:
42966610
Bibliographic data and abstract were imported from PubMed on 01 Nov 2026.

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