Authors
Xiya Liu, Jing Zhang, Weiyan Wang, Siyi Zheng, Juan Wang
Published in
Analytica chimica acta. Volume 1426. Pages 346355. Dec 08, 2026. Epub Oct 03, 2026.
Abstract
Intratumoral metabolic heterogeneity drives tumor progression and therapeutic resistance, yet reliable tools for in situ, real-time, and spatially resolved monitoring of intratumoral metabolites in living organisms remain scarce. Herein, a helical integrated hydrogel multiplex sensor (HIHMS) was fabricated for glucose monitoring within solid tumors. The HIHMS integrates three synergistic design elements into a unified sensing platform: (i) a helical multi-electrode architecture enabling simultaneous photoelectrochemical detection at three distinct spatial depths within tissues; (ii) glioblastoma cell membrane functionalization providing GLUT1-mediated selectivity for enzyme-free glucose detection in complex biological environments; and (iii) a dual PDMS/agarose coating strategy that eliminates inter-electrode crosstalk while maintaining biocompatibility for implantation. The sensor achieves a detection limit of 57 nM, a wide linear range of 0.1 μM-50 mM, and long-term in vivo monitoring over 28 days. Using this platform, we performed dynamic in vivo monitoring of intratumoral glucose spatial heterogeneity in tumor-bearing mice exposed to three widely used antibiotics (sulfadiazine, ciprofloxacin, amoxicillin) at gradient dosages. The results demonstrate that antibiotic exposure modulates glucose heterogeneity in a dose- and time-dependent manner: high-dose exposure exacerbates metabolic heterogeneity and accelerates tumor progression, while environmentally relevant concentrations induce an initial decrease followed by a sustained increase in heterogeneity.
PMID:
42967788
Bibliographic data and abstract were imported from PubMed on 02 Nov 2026.
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